MUSCLEMOTION: New software tool to measure cell contractions
Luc Sala et al published a software tool that can be used to measure cell contractions.
NB: This tool was also presented at the Interactive Masterclass with Kit Parker, 12 September 2017 in Amsterdam.
Contraction of muscle reflects its physiological state. Methods to quantify contraction are often complex, expensive and tailored to specific models or recording conditions, or require specialist knowledge for data extraction. Here we describe an automated, open-source software tool (MUSCLEMOTION) adaptable for use with standard laboratory and clinical imaging equipment that enables quantitative analysis of normal cardiac contraction, disease phenotypes and pharmacological responses.
MUSCLEMOTION allowed rapid and easy measurement of contractility
in (i) single cardiomyocytes from primary adult heart and human
pluripotent stem cells, (ii) multicellular 2D-cardiomyocyte
cultures, 3D engineered heart tissues and cardiac
organoids/microtissues in vitro and (iii) intact hearts of
zebrafish and humans in vivo. Good correlation was found with
conventional measures of contraction in each system. Thus, using a
single method for processing video recordings, we obtained reliable
pharmacological data and measures of cardiac disease phenotype in
experimental cell- and animal models and human echocardiograms.
The preprint is at http://www.biorxiv.org/content/early/2017/07/07/160754
The code can be downloaded at http://www.biorxiv.org/content/biorxiv/suppl/2017/07/07/160754.DC1/160754-3.txt